Rapid mycobacterium identification method based on nanopore sequencer

The invention discloses a rapid mycobacterium identification method based on a nanopore sequencer, belonging to the field of molecular biology. The rapid mycobacterium identification method comprises the following steps: step 1, designing specific primers for to-be-detected regions of mycobacterium tuberculosis insertion sequence IS6110, hsp65, rpoB, 16S rRNA and gyrB genes; step 2, acquiring primers containing specific base sequences, and mixing the primers to form a primer pool; step 3, synthesizing a Barcode primer; step 4, extracting genome DNA; step 5, performing first-round amplification and purification; step 6, carrying out second-round amplification and purification; step 7, constructing a sequencing library and carrying out purifying; step 8, conducting sequencing by using the nanopore sequencer, performing quality control analysis on data, and then performing data analysis. According to the invention, the amplification primers of the combination of the above five target genes is found, so an amplif