HBV detection based on CRISPR/Cas system

The invention provides brand-new HBV recognition targets (region 1 and region 2), and further provides brand-new crRNA. The sequence of the crRNA disclosed by the invention can be as shown in SEQ ID NO. 3 or SEQ ID NO. 4. The invention also provides a kit based on CRISPR, and the kit comprises any o...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	YUAN MINGZHU, DUAN GUANGCAI, YANG HAIYAN, XI YUANLIN, DING RONGHUA, JIN LEFEI, CHEN SHUAIYIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides brand-new HBV recognition targets (region 1 and region 2), and further provides brand-new crRNA. The sequence of the crRNA disclosed by the invention can be as shown in SEQ ID NO. 3 or SEQ ID NO. 4. The invention also provides a kit based on CRISPR, and the kit comprises any one of the crRNA. On the premise of ensuring the detection sensitivity, the detection technology can improve the HBV detection efficiency and reduce the detection cost, does not depend on professional equipment and technicians, and has the advantages of being easy to popularize and the like. 本发明提供了全新的HBV识别靶点region1、region2，还提供了全新的crRNA。本发明的crRNA，其序列可如SEQ ID NO.3或SEQ ID NO.4所示。本发明还提供了基于CRISPR的试剂盒，其包含任一所述crRNA。本专利检测技术在保证检测灵敏度的前提下，可提高HBV检测的效率，降低检测成本，并且不依赖专业设备和技术人员，具有易推广普及等优势。