Low-cost self-made microfluidic ELISA chip as well as preparation method and use method thereof

The invention discloses a low-cost self-made microfluidic ELISA chip as well as a preparation method and a use method thereof. The use method is simple, multiple indexes can be detected at the same time, and the reaction time is saved. The qualitative results can be obtained through naked eye observation, and quantitative processing can be performed by using a mobile phone to shoot images; the detection sensitivity is greatly improved, and positive signals of a to-be-detected sample which is diluted by 10000 times can still be correctly detected; the consumption of samples and reagents is reduced, expensive instruments do not need to be arranged, the use cost is greatly reduced, on-site instant detection becomes possible, and the product has predictable huge economic value and social value. 本发明公开了一种低成本可自制的微流控ELISA芯片及其制备方法和使用方法，使用方法简单，多指标可同时检测，节省反应时间。通过裸眼观察即可获取定性结果、使用手机拍摄图像即可定量处理；检测灵敏度大幅提高，对稀释10000倍的待测样本仍然能正确检出阳性信号；降低了样品和试剂消耗，并且不需要配备昂贵的仪器，极大地降低了使用成本，使现场即时检测成为可能，具有可预见的巨大的经济价值和社会价值。