Method for separating exosome from cerebrospinal fluid

The invention discloses a method for separating exosome from cerebrospinal fluid, belongs to the technical field of nano materials, and particularly relates to a method which comprises the following steps: modifying and carboxylating magnetic nanoparticles, and grafting with streptavidin to obtain functionalized magnetic nanoparticles; then capturing and separating the exosome in cerebrospinal fluid through functionalized magnetic nanoparticles, Tim4 protein and a calcium element; in the modification treatment, adding oleic acid, pteroic acid and alkanol lactate into magnetic nanoparticle dispersion liquid for modification treatment. The effective hydraulic diameter of the functionalized magnetic nanoparticles prepared by the method is large, and the grafting effect of streptavidin on the functionalized magnetic nanoparticles is good. The method has a good capture effect on the exosome in the cerebrospinal fluid, and the capture efficiency is 15-22%. 本发明公开了一种分离脑脊液中外泌体的方法，属于纳米材料技术领域，具体涉及通过对磁性纳米粒子改性处理以及羧基化处理后，再