Preparation method and application of streptochytrium limacinum induced resistance protein LiiP1

The invention discloses an application of streptochytrium limacinum induced resistance protein LiiP1 as a feed additive. The invention also discloses a preparation method of the streptochytrium limacinum induced resistance protein LiiP1. The preparation method comprises the following steps: step A,...

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Hauptverfasser:	QIU DEWEN, CHENG YUANLING, WANG SHIHAO, PENG ZHICHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FODDER FOODS OR FOODSTUFFS FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an application of streptochytrium limacinum induced resistance protein LiiP1 as a feed additive. The invention also discloses a preparation method of the streptochytrium limacinum induced resistance protein LiiP1. The preparation method comprises the following steps: step A, picking a small amount of streptochytrium limacinum thalli, inoculating the streptochytrium limacinum thalli into a shake flask liquid culture medium, and carrying out seed fermentation and shake culture; step B, inoculating the seed shake flask into a fermentation tank for at least two-stage fermentation; step C, after fermentation is completed, adding a buffer solution to protect induced resistance protein, and then carrying out wall breaking; and step D, inactivating protease in the crude protein liquid subjected to wall breaking of the thallus cells, concentrating the crude protein liquid, adding a carrier filler aid, granulating and drying the mixture to obtain the streptochytrium limacinum induced resistance