USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS
The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-...
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| creator | TRAVIS ALEXANDER KOHN ROLAND |
| description | The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3' end, (iii) a 5' tail, (iv) a target-specific portion between the 3' end and the 5' tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3' end or the 5' tail, and (vii) the 3' end and the 5' tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application.
本申请涉及检测样品中靶核酸分子的方法。所述方法包括提供含有靶核酸分子的样品和捕获寡核苷酸分子。在一个实施方案中，所述捕获寡核苷酸分子(i)长度为30-60个碱基对，(ii)在其3'端上具有4-8个碱基对的突出端，(iii)具有5'尾，(i |
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本申请涉及检测样品中靶核酸分子的方法。所述方法包括提供含有靶核酸分子的样品和捕获寡核苷酸分子。在一个实施方案中，所述捕获寡核苷酸分子(i)长度为30-60个碱基对，(ii)在其3'端上具有4-8个碱基对的突出端，(iii)具有5'尾，(i</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211112&DB=EPODOC&CC=CN&NR=113646439A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211112&DB=EPODOC&CC=CN&NR=113646439A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TRAVIS ALEXANDER</creatorcontrib><creatorcontrib>KOHN ROLAND</creatorcontrib><title>USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS</title><description>The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3' end, (iii) a 5' tail, (iv) a target-specific portion between the 3' end and the 5' tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3' end or the 5' tail, and (vii) the 3' end and the 5' tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application.
本申请涉及检测样品中靶核酸分子的方法。所述方法包括提供含有靶核酸分子的样品和捕获寡核苷酸分子。在一个实施方案中，所述捕获寡核苷酸分子(i)长度为30-60个碱基对，(ii)在其3'端上具有4-8个碱基对的突出端，(iii)具有5'尾，(i</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNALDfb0c1cIcQ3xcA1ydVFw9YuK9HUNVgjxV3BxDXF1DlHwC3X2cfV0VnB09nQJ5mFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hobGZiZmJsaWjsbEqAEA3AIlqA</recordid><startdate>20211112</startdate><enddate>20211112</enddate><creator>TRAVIS ALEXANDER</creator><creator>KOHN ROLAND</creator><scope>EVB</scope></search><sort><creationdate>20211112</creationdate><title>USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS</title><author>TRAVIS ALEXANDER ; KOHN ROLAND</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113646439A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TRAVIS ALEXANDER</creatorcontrib><creatorcontrib>KOHN ROLAND</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TRAVIS ALEXANDER</au><au>KOHN ROLAND</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS</title><date>2021-11-12</date><risdate>2021</risdate><abstract>The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3' end, (iii) a 5' tail, (iv) a target-specific portion between the 3' end and the 5' tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3' end or the 5' tail, and (vii) the 3' end and the 5' tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application.
本申请涉及检测样品中靶核酸分子的方法。所述方法包括提供含有靶核酸分子的样品和捕获寡核苷酸分子。在一个实施方案中，所述捕获寡核苷酸分子(i)长度为30-60个碱基对，(ii)在其3'端上具有4-8个碱基对的突出端，(iii)具有5'尾，(i</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS |
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