USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS

The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3' end, (iii) a 5' tail, (iv) a target-specific portion between the 3' end and the 5' tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3' end or the 5' tail, and (vii) the 3' end and the 5' tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application. 本申请涉及检测样品中靶核酸分子的方法。所述方法包括提供含有靶核酸分子的样品和捕获寡核苷酸分子。在一个实施方案中，所述捕获寡核苷酸分子(i)长度为30-60个碱基对，(ii)在其3'端上具有4-8个碱基对的突出端，(iii)具有5'尾，(i