Method for detecting organic and inorganic pollutants based on novel fluorescent sensor array
The invention discloses a method for detecting organic and inorganic pollutants based on a novel fluorescent sensor array. The method comprises the following steps: S1, preparing the fluorescent sensor array based on aptamer-sulfydryl double-sided specific recognition; s2, determining data of a seri...
Gespeichert in:
Hauptverfasser: | , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a method for detecting organic and inorganic pollutants based on a novel fluorescent sensor array. The method comprises the following steps: S1, preparing the fluorescent sensor array based on aptamer-sulfydryl double-sided specific recognition; s2, determining data of a series of concentration samples; s3, determining the types of organic and inorganic pollutants in an unknown sample: dispersing the Janus-r/g QDs-SH/Apt composite material prepared in the step S1 in a phosphate buffer solution with the pH value of 7, mixing with the unknown sample, incubating at 25 DEG C for 30 minutes, determining a fluorescence value, and performing parallel determination for 4 times to obtain a data matrix of 4 times of repeated * 2 signal output; and simultaneously drawing an LDA graph by using the data and the detection data of the series concentration samples, and judging whether the unknown sample contains a target object or not according to the overlapping or position relationship of data point |
---|