Method for detecting organic and inorganic pollutants based on novel fluorescent sensor array

The invention discloses a method for detecting organic and inorganic pollutants based on a novel fluorescent sensor array. The method comprises the following steps: S1, preparing the fluorescent sensor array based on aptamer-sulfydryl double-sided specific recognition; s2, determining data of a seri...

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Bibliographische Detailangaben
Hauptverfasser: LIU WANQIONG, CEN QIHANG, PENG JIANWEI, GUI CHENGYI, HUANG ZEYU, PENG ZHIHUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for detecting organic and inorganic pollutants based on a novel fluorescent sensor array. The method comprises the following steps: S1, preparing the fluorescent sensor array based on aptamer-sulfydryl double-sided specific recognition; s2, determining data of a series of concentration samples; s3, determining the types of organic and inorganic pollutants in an unknown sample: dispersing the Janus-r/g QDs-SH/Apt composite material prepared in the step S1 in a phosphate buffer solution with the pH value of 7, mixing with the unknown sample, incubating at 25 DEG C for 30 minutes, determining a fluorescence value, and performing parallel determination for 4 times to obtain a data matrix of 4 times of repeated * 2 signal output; and simultaneously drawing an LDA graph by using the data and the detection data of the series concentration samples, and judging whether the unknown sample contains a target object or not according to the overlapping or position relationship of data point