Micro-drop type digital PCR method for detecting fish nervous necrosis virus and corresponding kit

The invention belongs to the technical field of detection of fish nervous necrosis virus, and particularly relates to a micro-drop type digital PCR method for detecting fish nervous necrosis virus and a corresponding kit. In order to develop a nervous necrosis virus detection method with high sensitivity, the invention establishes the micro-drop type digital PCR method for detecting fish nervous necrosis virus, namely after a blood sample of a fish to be detected is collected, total RNA of the blood sample is extracted, and cDNA is synthesized; and then the cDNA is used as a template, and micro-drop type digital PCR is adopted, so that whether the fish to be detected is infected or carries NNV can be accurately detected. The micro-drop type digital PCR detection method provided by the invention is high in sensitivity, and has a good application prospect in breeding or selection of high-quality parents of cultured fishes. 本发明属于鱼类神经坏死病毒的检测技术领域，具体涉及用于检测鱼类神经坏死病毒的微滴式数字PCR方法及相应的试剂盒，为开发一种灵敏度较高的神经坏死病毒检测方法，本发明建立了一种用于检