Method and kit for detecting protein based on identification-induced isothermal amplification technology

The invention discloses a method for detecting protein based on an identification-induced isothermal amplification technology, which comprises the following steps: adding a functional template and a primer into a solution containing to-be-detected protein, and mixing, then adding DNA polymerase for amplification, and then adding a molecular beacon of which the 5' end is modified with a fluorophore and the 3' end is modified with a quenching group, a nicking incision enzyme I and a nicking incision enzyme II to obtain a reaction solution; directly subjecting the reaction liquid (amplification and cutting are still carried out) to fluorescence detection without incubation, carrying out real-time monitoring, and calculating the concentration of the protein according to a relational expression of fluorescence intensity and the concentration of the protein. The detection method has the advantages of no need of any separation, mild conditions (constant temperature of 37 DEG C), simple operation (two-step mixing), s