Preparation method of heterogeneity 3D tumor composite multicellular sphere invasion model

The invention discloses a preparation method of a heterogeneity 3D tumor composite multicellular sphere invasion model, and mainly solves the problems of high model preparation cost and high technical operation difficulty in the prior art. The method comprises the following steps of: 1) obtaining a single-cell suspension; 2) forming initial multiple tumor cells; 3) establishing a tumor cell strain capable of stably expressing a GFP fluorescent protein; 4) forming tumor composite multicellular cells; and 5) constructing the heterogeneity 3D tumor composite multicellular sphere invasion model. The invention successfully develops a simple, efficient and repeatable method for establishing the heterogeneity 3D tumor composite multicellular sphere invasion model, has a better condition for large-scale popularization, and also provides an important research model for the fields of research on tumor heterogeneity, tumor microenvironment, tumor immunity, tumor drug screening and the like, and the requirements of scien