Anti-CD117 protein monoclonal antibody and cell strain, preparation method and application thereof

The invention relates to a monoclonal antibody capable of recognizing a human CD117 antigen, a secretory cell strain, a preparation method of the monoclonal antibody and application of the secretory cell strain in immunodetection. According to the technical scheme, 950-976 amino acids at the C terminal of the CD117 protein are chemically synthesized as the antigen peptide, and the immunogen is obtained after the antigen peptide is coupled with the KLH. The CD117-KLH protein is used for immunizing a mouse, and through cell fusion, screening and subcloning, a mouse hybridoma cell strain 33G5 capable of efficiently secreting an anti-CD117 protein monoclonal antibody and the anti-CD117 protein monoclonal antibody secreted by the cell strain are obtained. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing CD117 protein, and is suitable for immunological detection, especially immunohistochemical detection. 本发明涉及一种可以识别人CD117抗原的单克隆抗体，分泌细胞株、其制备方法以及其在免疫