SERINE RECOMBINASES MEDIATING STABLE INTEGRATION INTO PLANT GENOMES

Described is a serine recombinase that integrates large sections of foreign DNA into plant chromosomes through non-homologous recombination through attP or attB sites on the foreign DNA but without the engineering of corresponding attB or attP site on the plant chromosome. The serine recombinase-based method has several advantages over other systems, such as Agrobacterium. Also described are related methods, composition, and plants containing exogenous DNA. 本发明描述了一种丝氨酸重组酶，其通过外来DNA上attP或attB位点的非同源重组将外来DNA的大区段整合到植物染色体中，而不需要对植物染色体上的相应attB或attP位点进行工程改造。基于丝氨酸重组酶的方法相比其他系统如农杆菌，有几个优点。还描述了相关方法、组合物和含有外源DNA的植物。