Composition for fixed-point modification of pAPN-gene 16 exon and application of composition

The invention relates to the technical field of biology and particularly provides a composition for fixed-point modification of a pAPN-gene 16 exon and application of the composition. The composition for the fixed-point modification of the pAPN-gene 16 exon, provided by the invention, comprises a targeted enzyme digestion vector 1, a targeted enzyme digestion vector 2 and a double-stranded donor sequence. A modified pAPN gene can effectively resist TGEV infection, meanwhile, the protein physiological activity function of the modified pAPN gene is not affected, and the modified pAPN gene has the advantages of being wide in application range, high in gene editing efficiency and the like. 本发明涉及生物技术领域，具体而言，提供了一种用于pAPN基因16外显子定点修饰的组合物及其应用。本发明提供的用于pAPN基因16外显子定点修饰的组合物，包括靶向酶切载体1、靶向酶切载体2和双链donor序列。改造后的pAPN基因可以有效抵抗TGEV感染，同时其蛋白生理活性功能不受影响，具有适用范围广、基因编辑效率高等优点。