Gene editing system for pAPN exon 16 and application thereof

The invention relates to the field of gene engineering, and particularly provides a gene editing system for pAPN exon 16 and application thereof. According to the gene editing system, and the sgRNA pair provided by the invention, endonuclease can be guided by a sgRNA pair to respectively target two target sites of the pAPN exon 16, and cutting editing is carried out, so that 69bp are deleted from the pAPN exon 16, and meanwhile, other amino acids of the pAPN gene are normally expressed. After pAPN gene editing, cells can effectively resist TGEV infection, other protein physiological activity functions are not affected, and the gene editing system has the advantages of being wide in application range, high in gene editing efficiency and the like. 本发明涉及基因工程领域，具体而言，提供了一种pAPN基因第16外显子的基因编辑系统及其应用。本发明提供的基因编辑系统，sgRNA对可以引导核酸内切酶分别靶向pAPN基因第16外显子的两个目标靶位点，并且进行切割编辑，实现pAPN基因第16外显子缺失69bp，同时pAPN基因的其余氨基酸正常表达。pAPN基因编辑后，细胞可以有效抵抗TGEV感染，同时其他蛋白生理活性功能不受影响，具有适用范围广、基因编辑效率高等优点。