Method for obtaining, functioning and regulating tissue stem cells

The invention discloses a method for obtaining, functioning and regulating tissue stem cells. The method comprises the following steps: 1, obtaining and culturing the tissue stem cells; 2, performing grouped induced differentiation on the tissue stem cells; 3, performing multi-group transplantation on differentiated cells; and 4, drawing a conclusion according to a comparison result. By comparing an adult mouse C with a young mouse D, the influence of a tissue microenvironment on tissue stem cell differentiation can be more clearly obtained, the gene sequence of the mouse is similar to that of a human body, the similarity between the whole genome of the mouse and the human body is extremely high, and the similar characters of many diseases which are difficult to cure by human beings can be found on the mouse, so that the method can be used for experimental research. 本发明公开了一种组织干细胞的获得、功能和调控方法，包括以下步骤：步骤1：组织干细胞获取和培养；步骤2：组织干细胞分组诱导分化；步骤3：分化细胞多组移植；步骤4：根据对比结果得出结论；通过采用成年小白鼠C和幼年小白鼠D进行对比，可以更加清晰的得出组织微环境对组织干细胞分化的影响，并且小白鼠的