Establishment method of cassava regeneration system

The invention discloses an establishment method of a cassava regeneration system, which comprises the following steps: step 1, obtaining cassava stem segments with axillary buds, and carrying out primary culture and subculture multiplication culture by using a first culture medium and a second culture medium until sterile leaves grow, wherein the first culture medium comprises an MS culture medium and 6-BA, and the second culture medium comprises 6-BA and NAA; step 2, taking the sterile leaves obtained in the step 1, and conducting callus culture through a third culture medium, wherein the third culture medium comprises the MS culture medium, Picloram and CuSO4; and step 3, taking callus obtained in the step 2, and carrying out induced differentiation and budding through a fourth culture medium, wherein the fourth culture medium comprises the MS culture medium, 6-BA and AgNO3. The establishment method can lay theoretical and technical foundations for target gene introduction in the cassava variety improvement