CDNA (complementary deoxyribonucleic acid) chip for screening and functional analysis of ginkgo genes

The invention relates to the technical field of cDNA chips, and provides a preparation method of a cDNA chip for screening and functional analysis of ginkgo genes, and the preparation method comprises the following steps: S1, selection of ginkgo leaves: selecting a plurality of ginkgo leaves suitabl...

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Bibliographische Detailangaben
Hauptverfasser: ZHU YE, CHEN YANYU, LIU CAOZHI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of cDNA chips, and provides a preparation method of a cDNA chip for screening and functional analysis of ginkgo genes, and the preparation method comprises the following steps: S1, selection of ginkgo leaves: selecting a plurality of ginkgo leaves suitable for preparing chips for later use; S2, preparing a ginkgo leaf disinfectant, and adding the ginkgo leaves in the S1 into the disinfectant for disinfection; S3, fishing out the ginkgo leaves in the S2, washing for later use, and then drying and slicing the washed ginkgo leaves; S4, extracting RNA (Ribonucleic Acid) from the ginkgo leaves in the S3, then carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), and presetting the cDNA in a PCR (Polymerase Chain Reaction) reaction plate to prepare a cDNA chip; and S5, selecting a plurality of samples from the step S3, and carrying out operation in the step S4 so as to obtain a plurality of cDNA chips. According to the method, a prefabr