Primer, probe and kit for detecting echinococcus

The invention discloses a primer, a probe and a kit for detecting echinococcus. The kit comprises an upstream primer of which the nucleotide sequence is shown as SEQ ID No.1, a downstream primer of which the nucleotide sequence is shown as SEQ ID No.2 and a probe of which the nucleotide sequence is shown as SEQ ID No.3, wherein a fluorescence reporter group and a fluorescence quenching group are respectively arranged at the 5'end and the 3 'end of the probe. The primer and the probe can specifically amplify and recognize the COX3 gene segment of the echinococcus, and the detected lowest concentration is 101 copy order of magnitude. The kit is rapid, efficient and sensitive, and can be suitable for any sample containing echinococcus, worm eggs or larvae. 本发明公开了一种检测棘球绦虫的引物、探针及其试剂盒。所述试剂盒包括核苷酸序列如SEQ ID No.1所示的上游引物和核苷酸序列如SEQ ID No.2所示的下游引物以及核苷酸序列如SEQ ID No.3所示的探针，所述探针的5'端和3'端分别设置有荧光报告基团和荧光淬灭基团。所述引物和探针能够特异性扩增及识别棘球绦虫COX3基因片段，且检出的最低浓度是101拷贝数量级。该试剂盒快速、高效、灵敏，能够适用于任何含有棘球绦虫、虫卵或幼虫的样本。