Reagent and kit for rapidly extracting nucleic acid from FFPE sample and application of reagent and kit

The invention belongs to the technical field of molecular biology, and particularly discloses a reagent and a kit for rapidly extracting nucleic acid from an FFPE sample and application of the reagent and the kit. The combination buffer solution in the reagent is simple in formula and low in cost, t...

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Hauptverfasser: YANG ZENGLI, ZHANG XIAOLIANG, CUI GUOPENG, ZHANG YIN, DONG CHAO
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the technical field of molecular biology, and particularly discloses a reagent and a kit for rapidly extracting nucleic acid from an FFPE sample and application of the reagent and the kit. The combination buffer solution in the reagent is simple in formula and low in cost, the combination effect of the nucleic acid and the magnetic beads can be remarkably improved, and the combination amount of the nucleic acid is increased. According to the method, the lysis buffer solution, the binding buffer solution and the washing buffer solution with specific components are combined for use, tissue cells are fully lysed, protein bound with nucleic acid is digested, the nucleic acid is completely released, then the released nucleic acid is specifically bound to the magnetic beads, the binding amount of the nucleic acid is increased, effective separation of the nucleic acid and other lysates is achieved, the magnetic beads combined with the nucleic acid are washed for multiple times, impurities su