Fermentation medium for producing gamma-aminobutyric acid and production method

The invention discloses a fermentation medium for producing gamma-aminobutyric acid. The fermentation medium for producing gamma-aminobutyric acid comprises the following components in percentage by weight: 0.1-1% of L-Glu, 10-20% of bean dregs, 0.05-0.2% of a K.marxianus leavening agent and the bal...

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Bibliographische Detailangaben
Hauptverfasser: YU HANSONG, YUE YANG, ZHANG LEI, DAI WEICHANG, PIAO CHUNHONG, WANG XIUJUAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a fermentation medium for producing gamma-aminobutyric acid. The fermentation medium for producing gamma-aminobutyric acid comprises the following components in percentage by weight: 0.1-1% of L-Glu, 10-20% of bean dregs, 0.05-0.2% of a K.marxianus leavening agent and the balance of water. The invention also relates to a production method of gamma-aminobutyric acid, which comprises the following steps: 1) culture of a K.marxianus leavening agent: growing a strain K.marxianus in an MRS culture medium, and carrying out shake cultivation for 10-15 hours at 28-32 DEG C and 100-150 rpm; centrifuging for 3 to 7 minutes at the speed of 5000 to 6000 rpm and the temperature of 0 to 4 DEG C; washing for three times, resuspending, and freeze-drying to obtain a K.marxianus leavening agent; 2) producing gamma-aminobutyric acid: mixing bean dregs, water and L-Glu, sterilizing at 121 DEG C for 20 minutes, and cooling to room temperature; inoculating a K.marxianus leavening agent, and fermenting for 4