Sperm washing upstream method

The invention belongs to the technical field of sperm optimization, and particularly relates to a sperm washing upstream method. The method comprises the following steps: (1) balancing a centrifugal tube A and a centrifugal tube B which are used for containing a fertilization culture solution in a CO2 incubator with the temperature of 37 DEG C and the volume ratio of 5% for 6-18 hours; (2) liquefying the semen at 25-37 DEG C for 30-60 minutes to obtain semen volume, sperm concentration, forward sperm percentage and forward sperm concentration; (3) adding the semen into the centrifugal tube B, and centrifuging; (4) adding part of the fertilization culture solution in the centrifugal tube A into a centrifugal tube B, and centrifuging; (5) adding the residual fertilization culture solution in the centrifugal tube A into the centrifugal tube B, and carrying upstream culture in a CO2 incubator with the temperature of 37 DEG C and the volume ratio of 5% for 20-30 minutes; (6) sucking out upstream liquid of the cent