Construction method, detection method and content determination method of China rose fingerprint spectrum

The invention relates to a construction method, a detection method and a content determination method of a China rose fingerprint spectrum. The construction method comprises the following steps: preparing a gallic acid reference substance solution, an ellagic acid reference substance solution, a hyperoside reference substance solution, an isoquercitrin reference substance solution, a quercitrin reference substance solution, a baicalin reference substance solution, a quercetin reference substance solution and an avicularin reference substance solution, a astragalus smicus glycoside reference substance solution and an afzelin reference substance solution; dissolving China rose, and carrying out ultrasonic treatment to prepare a test solution; and carrying out ultra-high performance liquid chromatography determination on each reference substance solution and the test solution, and establishing the China rose fingerprint spectrum. The established fingerprint spectrum effectively reflects qualitative characteristi