Tissue culture proliferation method of A.callosa var.pubiramula.C.Y.Wu

The invention discloses a tissue culture proliferation method of A.callosa var.pubiramula.C.Y.Wu. The tissue culture proliferation method comprises the following steps: selection of explants, sterilization of the explants, establishment of a sterile system and subculture proliferation. The selection of the explants comprises the following steps: in sunny weather from February to April, cutting a tender stem section of a current-year vegetative branch of the A.callosa var.pubiramula.C.Y.Wu; the sterilization of the explants comprises the following steps: cutting off leaves on tender stem, cleaning the stem section with washing powder, flushing with tap water for 0.5 h, cutting the stem section into the length of 2 cm, putting the cut stem sections into an ultra-clean workbench, soaking the stem sections with 75% alcohol for 30 s, flushing the alcohol with sterilized distilled water, putting the stem sections into a 0.1% mercury bichloride solution, soaking the stem sections for 3-20 min, and then cleaning with