Amoxicillin impurity positioning solution and preparation method thereof

The invention relates to the technical field of pharmaceutical analysis, in particular to an amoxicillin impurity positioning solution and a preparation method thereof. The preparation method comprises the following steps: dissolving first amoxicillin in an alkali solution, standing, carrying out first heating treatment under an acidic condition, and then adjusting to be neutral to obtain a first degradation solution; dissolving second amoxicillin in the neutral buffer solution, and carrying out second heating treatment to obtain a second degradation solution; and mixing the first degradation solution and the second degradation solution to obtain the amoxicillin impurity positioning solution. According to the present invention, the impurity positioning problem in the amoxicillin related substance detection can be well solved, the defect that the existing method is difficult to popularize due to the difficult purchase or the high price of the reference substance is overcome, and the good application prospect i