Method for simultaneously detecting cross-linked structures of nine lignin monomers in wheat stalks

The invention discloses a method for simultaneously detecting cross-linked structures of nine lignin monomers in wheat stalks, the cross-linked structures of the lignin monomers are S(8-O-4)S(8-8)S, S(8-O-4)G(8-O-4)S, G(8-O-4)S(8-5)G, S(8-O-4)S(8-5)G, G(8-O-4)S(8-8)S, G(8-O-4)G(8-5)G, S(8-O-4)G, G(8-8)G, G(8-5)H, UPLC-MS/MS is adopted, and chromatographic conditions are as follows: ultrapure water is taken as a mobile phase A, acetonitrile is taken as a mobile phase B, and gradient elution is performed. The method provided by the invention has the advantages of strong specificity, high sensitivity, high flux and objective and easy-to-analyze results, can be used for determining the change and the like of the crosslinking structure of the lignin monomer in the growth and development of the wheat stalks, and can also provide guidance for the decomposition of the lignin of the wheat stalks. 本发明公开了一种同时检测小麦茎秆中九种木质素单体交联结构的方法，所述木质素单体交联结构为S(8-O-4)S(8-8)S、S(8-O-4)G(8-O-4)S、G(8-O-4)S(8-5)G、S(8-O-4)S(8-5)G、G(8-O-4)S(8-8