EHDV-2 monoclonal antibody, preparation source cell strain, product application and kit

The invention relates to an EHDV-2 monoclonal antibody, a preparation source cell strain, a product application and a kit. The method comprises the following steps: extracting the epidemic hemorrhagic disease virus type 2 monoclonal antibody from a hybridoma cell strain EHD E2C5, injecting the monoclonal antibody into the abdominal cavity of a mouse, and purifying ascites. The antibody detection kit can be used for detection and epidemiological investigation of the epidemic hemorrhagic disease virus serum antibody, the operation procedure is simple, and the detection result and the technical performance index are equal to those of an EHD ELISA antibody detection method recommended by the world animal hygiene organization (OIE). 本发明涉及EHDV-2型单克隆抗体、制备源细胞株、产物应用及试剂盒。本发明方法包括以下步骤：在杂交瘤细胞株EHD E2C5中提取流行性出血病病毒2型单克隆抗体，注射小鼠腹腔，取腹水纯化制备而得。本发明中的抗体检测试剂盒可用于流行性出血病病毒血清抗体的检测和流行病学调查，操作程序简单，检测结果、技术性能指标均等同于世界动物卫生组织(OIE)推荐的EHD ELISA抗体检测方法。