Preparation method of specific antibody of paralichthys olivaceus mature dendritic cell marker molecule CD83

The invention provides a specific antibody for identifying mature dendritic cells of paralichthys olivaceus. The specific antibody is prepared by taking polypeptide with an amino acid sequence of SEQ ID NO: 1 as an antigen. The antigen epitope capable of preparing the specific antibody is obtained through screening, the prepared recombinant protein serves as an antigen to prepare the antibody, the antibody provided by the prepared antibody can identify the dendritic cells from the marker molecule level, and an important tool is provided for detecting the paralichthys olivaceus dendritic cells. 本发明提供一种用于鉴定牙鲆成熟树突状细胞的特异性抗体，是用氨基酸序列为SEQ ID NO:1的多肽作为抗原制备的。本发明筛选获得了能够制备特异性抗体的抗原表位，制备重组蛋白作为抗原来制备抗体，制备抗体所提供的抗体可以从标志分子层面对树突状细胞进行鉴定，为检测牙鲆树突状细胞提供了重要工具。