Ultrasonic pipetting device and method

The invention provides an ultrasonic pipetting device which comprises an ultrasonic transmitting module and a liquid carrying device, the ultrasonic transmitting module comprises a multi-probe assembly, a coupling liquid circulating system and an ultrasonic excitation assembly, and the liquid carrying device is arranged on the multi-probe assembly; the multi-probe assembly comprises two or more probes, a coupling cavity and a coupling nozzle, the two or more probes are arranged on the same support, a plurality of liquid carrying holes are formed in the liquid carrying device, the focal points of the probes are aligned with the liquid level of the liquid carrying device, the coupling cavity surrounds the probes, the coupling nozzle is arranged between the probes and the liquid carrying device, and the coupling cavity is filled with coupling liquid; the coupling liquid circulating system enables coupling liquid to continuously enter the coupling cavity, and the coupling liquid overflows from the coupling nozzle