Primer and method for fluorescent quantitative PCR detection of Enterocytozoon hepatopenaei (EHP)

The invention relates to a method for fluorescent quantitative PCR detection of Enterocytozoon hepatopenaei (EHP). According to the method, a pair of specific primers taking EHP spore wall protein SWP as a target gene and an SWP standard substance kit are used. The standard substance kit comprises an SWP standard plasmid diluted by 10 times of gradient and a bacterial suspension of an SWP standard strain. A TBGreen fluorescent dye method is adopted, the EHP infection of cultured prawns is quantitatively detected by detecting the EHP SWP, high sensitivity and specificity are achieved, the method can be used for rapid detection and diagnosis of EHP, and a foundation is laid for monitoring and early prevention and control of EHP infection. 本发明涉及一种用于检测对虾肝肠孢虫的荧光定量PCR方法，该方法包括一对以虾肝肠孢虫孢壁蛋白SWP为靶基因的特异性引物，以及SWP标准品试剂盒。标准品试剂盒包括10倍梯度稀释的SWP标准质粒以及SWP标准菌株的菌悬液。本发明采用TBGreen荧光染料法，通过检测虾肝肠孢虫孢壁蛋白SWP,来定量检测养殖对虾肝肠孢虫感染，具有较高的灵敏度和特异性，可用于虾肝肠孢虫的快速检测和诊断，为虾肝肠孢虫感染的监测和早期防控奠定基础。