Vaccine immunopotentiator recombinant IFN (interferon) gamma, IL-1beta and TNF (tumor necrosis factor) alpha as well as preparation method and application thereof

The invention provides vaccine immunopotentiators recombinant IFN gamma, IL-1beta and TNF alpha as well as a preparation method and application thereof, and belongs to the technical field of fish molecular immunology. Research finds that pIgR and IgM gene expression and protein expression can be significantly up-regulated and the relative survival rate of grass carp can be significantly increased by combining the prepared IFN gamma and IL-1beta recombinant protein with inactivated Flavobacterium columnar (FC), so that the IFN gamma and IL-1beta recombinant protein can be used as a grass carp vaccine immunopotentiator, has the advantages of no harmful residue and the like after being used, is very suitable for natural ecological and environment-friendly industrial standards advocated by the current industry, has important theoretical and practical significance for prevention and treatment of grass carp diseases, and also has important reference value for prevention and treatment of diseases of other cultured f