Automated biomanufacturing systems, facilities, and methods

Disclosed are a method and automated facility for manufacturing a purified protein of interest using one or more single-use perfusion bioreactors, one or more single-use surge vessels and one or more chromatography systems. The protein of interest can be a recombinant or naturally occurring protein and/or a therapeutic or other medically useful protein. For example, the disclosed method and automated facility are useful for manufacturing a purified protein drug substance. 公开了用于生产纯化的目标蛋白质的方法和自动化设备，所述方法和自动化设备使用一种以上一次性灌注生物反应器、一种以上一次性缓冲容器和一种以上色谱系统。目标蛋白质可以为重组的或天然存在的蛋白质和/或治疗性或其他医学上有用的蛋白质。例如，所公开的方法和自动化设备可用于生产纯化的蛋白质药物。