Novel method for detecting enterobacter sakazakii, application and detection kit

The invention discloses a novel method for detecting enterobacter sakazakii, application and a detection kit. According to the method, enterobacter sakazakii pollution in dairy products is detected by analyzing the following characteristic peptides: I1: SEQ ID NO.1; I2: SEQ ID NO.2; I3: SEQ ID NO.3; I4: SEQ ID NO. 4. According to the method, a liquid chromatography triple quadrupole mass spectrometer is used for detecting the characteristic peptide. The detection process of the method does not depend on bacterial colony selection and biochemical identification, the method has high reliability, the detection time is greatly shortened, and a reliable and effective method is provided for detecting enterobacter sakakaakii pollution in food. 本发明公开了一种检测阪崎肠杆菌的新方法、应用及检测试剂盒，所述方法通过分析以下特征肽检测奶制品中的阪崎肠杆菌污染：I1：SEQ ID NO.1；I2：SEQ ID NO.2；I3：SEQ ID NO.3；I4：SEQ ID NO.4。本发明使用液相色谱三重四级杆质谱联用仪检测特征肽，本方法检测过程不依赖于菌落挑选和生化鉴定，具有高可靠性，并大大减少了检测时间，为食品中坂崎肠杆菌污染的检验提供了可靠有效的方法。