Method for on-line high-throughput detection of aflatoxin in corn kernels

The invention discloses a method for on-line high-throughput detection of aflatoxin in corn kernels. A device for realizing the method comprises a lamp belt, an objective lens, an imaging spectrometer, an ultra-high sensitivity camera, the corn kernels, a computer and a conveyor belt. Exciting light generated by the lamp belt irradiates the corn kernels thrown by the conveyor belt, the corn kernels containing aflatoxin generate fluorescence, a fluorescence signal passes through an objective lens and passes through an imaging spectrometer to be subjected to partial light, then the fluorescence signal is collected by an ultrahigh-sensitivity camera, a 410-560 nm wave band is selected in the collected wavelength range, 30 wavelengths are selected at equal intervals in the 410-560 nm wave band and transmitted to the computer, the computer fits a curve according to 30 wavelength points, the intensity integral of the wave band of 410-485nm and the intensity integral of the wave band of 485-560nmare respectively cal