Mouse esophageal squamous carcinoma cell line as well as establishment method and application thereof

The invention belongs to the technical field of tumor cell lines, and particularly relates to a mouse esophageal squamous carcinoma cell line as well as an establishment method and application thereof. The method comprises the following steps: 1) establishing a mouse orthotopic esophageal squamous cell carcinoma model: inducing cancerization of esophageal mucosa cells of a mouse by adopting a chemical reagent; 2) culturing a mouse esophageal squamous carcinoma cell line: cleaning the tumor tissue with a serum-free medium, cutting the tumor tissue into blocks, placing the blocks in a DMEM culture medium containing 20% FBS, and culturing at constant temperature; and continuously culturing and cleaning tissue blocks with cells climbing out, adding pancreatin for digestion, centrifuging, taking supernatant, re-suspending the cell precipitate, re-paving the cell precipitate in a culture bottle, and culturing to obtain the mouse esophageal squamous cell carcinoma stable cell line. According to the method, the mouse