Screening method of Marc-145 cell strain capable of being used for culturing PRRSV to obtain virus liquid with high virus content

The invention discloses a screening method of a Marc-145 cell strain capable of being used for culturing PRRSV to obtain a virus liquid with high virus content, and belongs to the technical field of bioengineering. The method provided by the invention comprises the steps: detecting the percentage of cells with CD163 receptors on the cell surfaces in a Marc-145 monoclonal cell population formed by breeding a candidate Marc-145 monoclonal cell strain in the Marc-145 cell population by utilizing an indirect immunofluorescence method, and screening out the Marc-145 cell strain which can be used for culturing PRRSV to obtain the virus liquid with high virus content according to the percentage. The method has the advantages of rapidness, convenience, objectiveness and accuracy, can predict the virus content in the virus liquid obtained by culturing PRRSV by the Marc-145 cell population, and can guide vaccine production. 本发明公开一种能够用于培养PRRSV获得高病毒含量病毒液的Marc-145细胞株的筛选方法，属于生物工程技术领域。本发明提供的方法包括利用间接免疫荧光法检测由候选Marc-145单克隆细胞株繁