Polypeptide V2 specifically bound with vibrio parahaemolyticus and application thereof

The invention discloses a polypeptide V2 specifically bound with vibrio parahaemolyticus and application thereof. The nucleotide sequence of the polypeptide V2 is shown as SEQ ID NO.2 in a sequence table. The polypeptide V2 and the application have the beneficial effects that (1) the polypeptide whi...

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Hauptverfasser: ZHAO CHAO, WANG JUAN, LI LI, ZHANG HUIWEN, CHEN FENG, YUAN BOCHAO, LI JUAN, ZHAI YUE, HAN XUEFEI, SHI XUENING, SONG XIULING, QU SHIFANG, LIU YUSHEN, XU KUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a polypeptide V2 specifically bound with vibrio parahaemolyticus and application thereof. The nucleotide sequence of the polypeptide V2 is shown as SEQ ID NO.2 in a sequence table. The polypeptide V2 and the application have the beneficial effects that (1) the polypeptide which is screened by a phage display technology and is specifically bound with the vibrio parahaemolyticus and the preparation and synthesis of a immunomagnetic compound are reported for the first time at home and abroad; (2) the enrichment efficiency of the immunomagnetic compound provided by the invention is as high as 93.52%; and (3) separation and enrichment of vibrio parahaemolyticus can be realized within 30 minutes by using the immunomagnetic compound provided by the invention, and compared with the traditional culture enrichment method, the time is greatly saved. 本发明公开了一种副溶血性弧菌特异性结合的多肽V2及其应用,它的核苷酸序列如序列表SEQ ID NO.2所示;它的有益效果是:(1)将噬菌体展示技术筛选出的与副溶血性弧菌特异性结合的多肽,并制备合成免疫磁性复合物为国内外首次报道;(2)利用本发明提供的免疫磁性复合物富集效率高达93.52%;(3)利