Immobilization method of free enzyme

The invention discloses an immobilization method of a free enzyme. According to the method, a microemulsion suspension technology is adopted for the first time, the free enzyme is adsorbed on a covalently modified inorganic carrier in situ, and the defects that the free enzyme is not easy to separate in the catalytic process, the traditional immobilized enzyme is poor in structural stability, the unit utilization efficiency of the enzyme is low and the like are effectively overcome. The loading capacity of the obtained immobilized enzyme reaches 200mg/g, the size of the enzyme is nanoscale, the average diameter is 3.5-6.5nm, the dispersion is uniform, and the agglomeration phenomenon is avoided. In addition, the process does not need a harsh preparation environment, the enzyme activity retention rate can reach 100%, and the mechanical loss rate is less than 0.05%. The method is suitable for synthesizing enzymes for catalyzing macromolecules and micromolecules, and has universality. 本发明公开了一种游离酶的固定化方法，该方法首次采用微乳