Method for rapidly recovering punicalagin from pomegranate rind extraction waste based on solvent effect

The invention relates to a method for rapidly recovering punicalagin from pomegranate rind extraction waste based on a solvent effect. According to the method, based on a solvent effect, methanol water, acetonitrile water or formic acid water is used as a dispersing solvent to disperse a precipitate...

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Hauptverfasser: AJIAIKEBAIER AISA, SUN GUANGYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for rapidly recovering punicalagin from pomegranate rind extraction waste based on a solvent effect. According to the method, based on a solvent effect, methanol water, acetonitrile water or formic acid water is used as a dispersing solvent to disperse a precipitate (namely pomegranate rind extraction waste) generated in a precipitation pretreatment process of a pomegranate ring extract, and then a sample is injected to a reversed-phase chromatographic column for separation; and a solvent effect is formed in the separation process by controlling the volume and the elution strength of a sample injection solvent and the elution strength of a mobile phase. Large-polarity punicalagin is rapidly eluted at a solvent peak, and small-polarity impurities are retained on a reversed-phase chromatographic column, so that a separation effect is achieved. According to the method, the pomegranate rind waste with the punicalagin content of 12% can be rapidly purified into a punicalagin-rich