RAPD reaction system and kit for euonymus bungeanus

The invention provides an RAPD reaction system for euonymus bungeanus, and belongs to the technical field of biological molecular markers. The total amount of the RAPD reaction system is 20 [mu] L, and the RAPD reaction system comprises the following components of 25-30 ng of template DNA, 0.15-0.25 [mu] mol/L of a primer, 2.50-2.70 mmol/L of MgCl2, 0.10-0.15 mmol/L of dNTP, 1.75-2.50 U of Taq DNA polymerase, and the balance of ddH2O. A strip amplified by the RAPD reaction system for the euonymus bungeanus is clear, complete and high in stability, and the blank of research on genetic diversity of the euonymus bungeanus in China is filled. 本发明提供了一种桃叶卫矛的RAPD反应体系，属于生物学分子标记技术领域，所述RAPD反应体系总量为20μL，包括如下含量组分：模板DNA25～30ng，引物0.15～0.25μmol/L，MgCl22.50～2.70mmol/L，dNTP 0.10～0.15mmol/L，Taq DNA聚合酶1.75～2.50U，余量为ddH2O。本发明建立的桃叶卫矛的RAPD反应体系扩增出的条带清晰、完整，稳定性高，弥补了国内对桃叶卫矛遗传多样性研究的空白。