Construction method of double-target fusion protein plasmid simultaneously combining TGF beta and VEGF

The invention relates to a construction method of a plasmid capable of expressing a fusion protein simultaneously targeting TGF beta and VEGF. The construction method comprises the following steps of: designing and constructing the fusion protein, synthesizing VEGFR-1 and TbetaR-II genes and primers, and carrying out PCR amplification; designing a primer by taking a pCI/Y2 plasmid as a template, and carrying out PCR amplification to obtain an Fc gene; obtaining a TF fusion gene segment from the Fc gene segment through a recombinant PCR method; carrying out Nhe I and EcoR I double enzyme digestion on the TF fusion gene segment, carrying out double enzyme digestion on a plasmid pIRESpuro3 by using the same group of enzyme, recovering the two enzyme digestion segments, and carrying out a ligation reaction to obtain a plasmid pIRESpuro3/TF; and carrying out BspE I and EcoR I double enzyme digestion on the successfully constructed pIRESpuro3/TF plasmid, carrying out double enzyme digestion on the obtained fusion g