Novel chitinase Chi3002 and application thereof

The invention provides a novel chitinase. The amino acid sequence of the novel chitinase is as shown in SEQ ID NO: 1. The chitinase provided by the invention is used for preparation. The novel vibrio chitinase provided by the invention is good in thermal stability, and the residual enzyme activity a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YU MIN, RAN LINGMAN, LI XI, ZHANG XIAOHUA
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a novel chitinase. The amino acid sequence of the novel chitinase is as shown in SEQ ID NO: 1. The chitinase provided by the invention is used for preparation. The novel vibrio chitinase provided by the invention is good in thermal stability, and the residual enzyme activity at 80 DEG C is kept at 80% or above. The pH tolerance range is wide, the residual enzyme activity is kept at 80% or above under the condition that the pH is 4-12, and the high application value is achieved. Moreover, when the chitinase is used for degrading chitin, the degradation product is mainly chitobiose. 本发明提供一种新型几丁质酶,其氨基酸序列为SEQ ID NO:1。本发明所提供的几丁质酶用于制备。本发明所提供的新型弧菌几丁质酶的热稳定性好,在80℃下残余酶活力保持在80%以上。其pH耐受范围较广,在pH 4-12条件下,残余酶活力保持在80%以上,有较高的应用价值。而且,其在降解几丁质时,降解产物主要为几丁二糖。