Method for preserving cucumber embryos

The invention relates to the technical field of cucumber tissue culture, in particular to a method for preserving cucumber embryos. Cucumber embryogenic callus and embryoid are recovered to grow and regenerated into plants after being preserved for a certain time. The preservation method comprises t...

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Hauptverfasser: XUE WANYU, CHEN SHUXIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of cucumber tissue culture, in particular to a method for preserving cucumber embryos. Cucumber embryogenic callus and embryoid are recovered to grow and regenerated into plants after being preserved for a certain time. The preservation method comprises the steps: the cucumber embryogenic callus and embryoid are cultured on a preservation culture medium, the preservation culture medium is based on an MS culture medium, 60 g/L cane sugar, 1.5 mg/L 2, 4-D and 7% agar need to be added, and the PH is 5.8. The specific culture medium is screened out, and on the culture medium, the cucumber embryogenic callus and embryoid are not prone to browning and can grow and be generated into normal plantlets. Generally, the cucumber embryogenic callus and embryoid can continuously develop and grow and gradually age to death, and according to the method, tissue aging is prevented by adjusting the concentration proportion of sucrose, the embryogenic callus and embryoid can be preser