Bovine sperm treatment method and bovine intracytoplasmic sperm injection method

The invention relates to a bovine sperm treatment method and a bovine intracytoplasmic sperm injection method, and belongs to the technical field of intracytoplasmic sperm injection. According to the bovine sperm treatment method, firstly, the sperm is treated by using a weakly alkaline solution, an...

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Hauptverfasser: ZHU XIAOTING, XIN XIAOLING, SHI QIAOTING, LYU SHIJIE, FENG YAJIE, ZHANG ZIJING, HUA LIUSHUAI, ZHAO CAIYAN, JIN LEI, WANG ERYAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a bovine sperm treatment method and a bovine intracytoplasmic sperm injection method, and belongs to the technical field of intracytoplasmic sperm injection. According to the bovine sperm treatment method, firstly, the sperm is treated by using a weakly alkaline solution, and therefore, the tail part of the sperm is shortened by 1/3-1/2; and then, the sperm of which the tail part is shortened by 1/3-1/2 is selected and is injected into the oocytes. After the bovine sperm treatment method disclosed by the invention is adopted for carrying out the treatment, only the tail part of the sperm is shortened by 1/3-1/2, the sperm still retains the centrosome, the integrity of the sperm is structurally retained, the method is more similar to the change of sperm capacitation in a natural fertilization process, the embryonic development potential is improved, and the development rate of microinjection embryos is improved. In addition, the tail part of the sperm treated by the bovine sperm treatm