Preparation method of biological membrane as well as product and application of biological membrane

The invention provides a preparation method of a biological membrane as well as a product and application of the biological membrane. The preparation method of the biological membrane comprises the following steps: (1) treating animal cavity tissues by adopting a collagenase inhibitor; (2) removing a membrane layer which accounts for more than 40% of the total fat content of animal cavity tissues; (3) treating a product obtained in the step (2) by adopting a first enzyme solution; (4) treating a product obtained in the step (3) by adopting an alkali solution; (5) treating a product obtained in the step (4) by adopting a decellularized solution; (6) treating a product obtained in the step (5) by adopting a degreasing fluid solution; (7) treating a product obtained in the step (6) by adopting a second enzyme solution; and (8) cleaning the product obtained in the step (7) by adopting supercritical carbon dioxide to obtain the biological membrane. The integrity of a multi-layer space structure, elastic fibers and