Novel telomerase-triggered DNA nano machine and construction method thereof

The invention discloses a telomerase-triggered DNA nano machine and a construction method thereof, which comprises gold nanoparticles, a probe mechanical arm sequence S1, a telomerase primer sequence S2, an ATP aptamer sequence S3 and an antisense DNA sequence S4, the sulfydryl at the 5' end of...

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Bibliographische Detailangaben
Hauptverfasser:	SHI YING, REN CHUNNIAN, LI YING, CHENG SIMIN, FU ZHUOLIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a telomerase-triggered DNA nano machine and a construction method thereof, which comprises gold nanoparticles, a probe mechanical arm sequence S1, a telomerase primer sequence S2, an ATP aptamer sequence S3 and an antisense DNA sequence S4, the sulfydryl at the 5' end of the probe mechanical arm sequence S1 is connected to the gold nanoparticles, a part of the sequence, close to the 3' end, of the probe mechanical arm sequence S1 forms an annular structure, the telomerase primer sequence S2 and the probe mechanical arm sequence S1 are complementarily hybridized to form a stem-loop structure, the sulfydryl at the 3' end of the ATP aptamer sequence S3 is connected to the gold nanoparticles, the antisense DNA sequence S4 and the ATP aptamer sequence S3 are complementarily hybridized, the 3' end of the antisense DNA sequence S4 is modified with a photosensitizer, and the 5' end of the ATP aptamer sequence S3 is modified with a dual-mode imaging group. Telomerase in tumor cells triggers the