Aromatic isopentenyl transferase mutant, construction method of recombinant bacteria for expression of aromatic isopentenyl transferase mutant and recombinant bacteria constructed by aromatic isopentenyl transferase mutant

The invention relates to an aromatic isopentenyl transferase mutant, a construction method of a recombinant bacterium for expression of the aromatic isopentenyl transferase mutant and the recombinant bacterium constructed by the aromatic isopentenyl transferase mutant. Specifically, the amino acid sequence of the aromatic isopentenyl transferase mutant has one or more of the following mutations relative to the amino acid sequence as shown in SEQ ID No.28: i) replacing the 161 < th > position Q of the sequence as shown in SEQ ID No.28 with A, N, S or V, ii) replacing the 286 < th > position G of the sequence as shown in SEQ ID No.28 with N, S or V, and iii) replacing the 288 < th > position Y of the sequence as shown in SEQ ID No.28 with A, N, S or V.The recombinant bacterium constructed by the invention has strong potential of industrial production of cannabinoid phenolic acid and derivatives thereof by efficiently expressing the aromatic isopentenyl transferase mutant. 本发明涉及芳香族异戊烯基转移酶突变体、用于其表达的重组菌的构建方法及由其构建的