Novel chimera TsCas12a protein and preparation technology

The invention discloses a novel chimera TsCas12a protein sequence and a preparation technology thereof. The research finds that a novel chimera Cas12a protein which specifically recognizes a target sequence under the mediation of gRNA and has non-specific single-stranded DNA cleavage activity serves as a novel CRISPR/TsCas12a system to be applied to nucleic acid detection, and can normally work in a reaction system containing high-concentration PEG and multiple nonionic detergents and reducing agents, and a new necessary tool choice is provided for one-step method molecular detection based on Cas12a. 本发明公开了一种新型嵌合体TsCas12a蛋白序列及制备技术。本发明研究发现一种在gRNA介导下对靶标序列特异性识别并具有非特异单链DNA切割活性的新型嵌合体Cas12a蛋白，作为新型CRISPR/TsCas12a系统应用于核酸检测，可在含有高浓度PEG、多种非离子型去垢剂和还原剂的反应体系中正常工作，为基于Cas12a的一步法分子检测提供了一种新的必要工具选择。