Recombinant cell line for efficiently and stably expressing feline interferon-omega2 and application of recombinant cell line

The invention provides a recombinant cell line for efficiently and stably expressing feline interferon-omega2 (FeIFN-omega2). In the recombinant cell line, the FeIFN-omega2 gene is stably inserted into a short arm 10 region (1p10) of a CHO cell chromosome 1, the CHO cell chromosome 1p10 is a strong transcriptional activity region, and compared with the insertion in a non-transcriptional activity region, the FeIFN-omega2 realizes stable and efficient expression. The FeIFN-omega generated by the recombinant cell line obtained by the invention has very strong antiviral biological activity, and is suitable for being used for preparing active ingredients of drugs for treating viral infectious diseases of dogs and cats. 本发明提供了一种高效稳定表达猫干扰素-ω2(FeIFN-ω2)的重组细胞系。所述重组细胞系中，FeIFN-ω2基因稳定插入在CHO细胞1号染色体短臂10区(1p10)，所述CHO细胞染色体1p10为强转录活性区，与插入在非转录活性区比较，FeIFN-ω2实现了稳定和高效表达。由本发明获得的重组细胞系产生的FeIFN-ω具有很强的抗病毒生物活性，适合用于制备治疗犬、猫病毒性感染性疾病药物的活性成分。