Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications
The invention discloses real-time fluorescent RAA primers and probes for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses, a kit including the primers and probes, and applications. The sensitivity of the method is 102 copies/reaction; and each reaction may sim...
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| creator | CAO CHENFU WU JIANG SHI WEIJUN LIN YANXING HUANG CHAOHUA HUA QUNYI ZENG SHAOLING YANG JUNXING RUAN ZHOUXI LIU JIANLI |
| description | The invention discloses real-time fluorescent RAA primers and probes for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses, a kit including the primers and probes, and applications. The sensitivity of the method is 102 copies/reaction; and each reaction may simultaneously and minimally detect 102 copies of porcine circovirus type 2 viruses and 102 copies of porcine circovirus type 3 viruses. Therefore, the porcine circovirus type 2 and porcine circovirus type 3 viruses can be rapidly, efficiently, sensitively and simultaneously detected; and effective technological means can be provided for rapid identification detection of mixed infection.
本发明公开了一种可同时快速检测猪圆环病毒2型和3型病毒的实时荧光RAA引物和探针、包括所述引物和探针的试剂盒及用途。本发明方法敏感性为102拷贝/反应，每个反应可同时最低检测出102拷贝的猪圆环病毒2型和102拷贝的猪圆环病毒3型病毒。本发明能快捷、高效、灵敏的同时检测猪圆环病毒2型和猪圆环病毒3型病毒，为两者的混合感染的快速鉴别检测提供了有效技术手段。 |
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本发明公开了一种可同时快速检测猪圆环病毒2型和3型病毒的实时荧光RAA引物和探针、包括所述引物和探针的试剂盒及用途。本发明方法敏感性为102拷贝/反应，每个反应可同时最低检测出102拷贝的猪圆环病毒2型和102拷贝的猪圆环病毒3型病毒。本发明能快捷、高效、灵敏的同时检测猪圆环病毒2型和猪圆环病毒3型病毒，为两者的混合感染的快速鉴别检测提供了有效技术手段。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210831&DB=EPODOC&CC=CN&NR=113322350A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25566,76549</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210831&DB=EPODOC&CC=CN&NR=113322350A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAO CHENFU</creatorcontrib><creatorcontrib>WU JIANG</creatorcontrib><creatorcontrib>SHI WEIJUN</creatorcontrib><creatorcontrib>LIN YANXING</creatorcontrib><creatorcontrib>HUANG CHAOHUA</creatorcontrib><creatorcontrib>HUA QUNYI</creatorcontrib><creatorcontrib>ZENG SHAOLING</creatorcontrib><creatorcontrib>YANG JUNXING</creatorcontrib><creatorcontrib>RUAN ZHOUXI</creatorcontrib><creatorcontrib>LIU JIANLI</creatorcontrib><title>Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications</title><description>The invention discloses real-time fluorescent RAA primers and probes for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses, a kit including the primers and probes, and applications. The sensitivity of the method is 102 copies/reaction; and each reaction may simultaneously and minimally detect 102 copies of porcine circovirus type 2 viruses and 102 copies of porcine circovirus type 3 viruses. Therefore, the porcine circovirus type 2 and porcine circovirus type 3 viruses can be rapidly, efficiently, sensitively and simultaneously detected; and effective technological means can be provided for rapid identification detection of mixed infection.
本发明公开了一种可同时快速检测猪圆环病毒2型和3型病毒的实时荧光RAA引物和探针、包括所述引物和探针的试剂盒及用途。本发明方法敏感性为102拷贝/反应，每个反应可同时最低检测出102拷贝的猪圆环病毒2型和102拷贝的猪圆环病毒3型病毒。本发明能快捷、高效、灵敏的同时检测猪圆环病毒2型和猪圆环病毒3型病毒，为两者的混合感染的快速鉴别检测提供了有效技术手段。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNikEKwjAQAHvxIOof1gcINsEHSFEEwZP3ErZbWYibmN0I_b1QvAqehmFm2byubDCmAsrPGi0IpapxgoGM0FgekFNBFgLkgunNpSrYlAkcBBl-Vg-zkM5XyDkyBuMkum4WY4hKmy9XzfZ8uneXHeXUk-aAJGR9d2tb753zh_3R__N8APl8RC0</recordid><startdate>20210831</startdate><enddate>20210831</enddate><creator>CAO CHENFU</creator><creator>WU JIANG</creator><creator>SHI WEIJUN</creator><creator>LIN YANXING</creator><creator>HUANG CHAOHUA</creator><creator>HUA QUNYI</creator><creator>ZENG SHAOLING</creator><creator>YANG JUNXING</creator><creator>RUAN ZHOUXI</creator><creator>LIU JIANLI</creator><scope>EVB</scope></search><sort><creationdate>20210831</creationdate><title>Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications</title><author>CAO CHENFU ; WU JIANG ; SHI WEIJUN ; LIN YANXING ; HUANG CHAOHUA ; HUA QUNYI ; ZENG SHAOLING ; YANG JUNXING ; RUAN ZHOUXI ; LIU JIANLI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113322350A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CAO CHENFU</creatorcontrib><creatorcontrib>WU JIANG</creatorcontrib><creatorcontrib>SHI WEIJUN</creatorcontrib><creatorcontrib>LIN YANXING</creatorcontrib><creatorcontrib>HUANG CHAOHUA</creatorcontrib><creatorcontrib>HUA QUNYI</creatorcontrib><creatorcontrib>ZENG SHAOLING</creatorcontrib><creatorcontrib>YANG JUNXING</creatorcontrib><creatorcontrib>RUAN ZHOUXI</creatorcontrib><creatorcontrib>LIU JIANLI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CAO CHENFU</au><au>WU JIANG</au><au>SHI WEIJUN</au><au>LIN YANXING</au><au>HUANG CHAOHUA</au><au>HUA QUNYI</au><au>ZENG SHAOLING</au><au>YANG JUNXING</au><au>RUAN ZHOUXI</au><au>LIU JIANLI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications</title><date>2021-08-31</date><risdate>2021</risdate><abstract>The invention discloses real-time fluorescent RAA primers and probes for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses, a kit including the primers and probes, and applications. The sensitivity of the method is 102 copies/reaction; and each reaction may simultaneously and minimally detect 102 copies of porcine circovirus type 2 viruses and 102 copies of porcine circovirus type 3 viruses. Therefore, the porcine circovirus type 2 and porcine circovirus type 3 viruses can be rapidly, efficiently, sensitively and simultaneously detected; and effective technological means can be provided for rapid identification detection of mixed infection.
本发明公开了一种可同时快速检测猪圆环病毒2型和3型病毒的实时荧光RAA引物和探针、包括所述引物和探针的试剂盒及用途。本发明方法敏感性为102拷贝/反应，每个反应可同时最低检测出102拷贝的猪圆环病毒2型和102拷贝的猪圆环病毒3型病毒。本发明能快捷、高效、灵敏的同时检测猪圆环病毒2型和猪圆环病毒3型病毒，为两者的混合感染的快速鉴别检测提供了有效技术手段。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for simultaneously detecting porcine circovirus type 2 and porcine circovirus type 3 viruses and applications |
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